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Purification of Genistein and Daidzein from Pueraria Lobata(Wild.) Ohwi by Coordination Chromatography

Tao Xu

Abstract

To establish a protocol for the purification of genistein and daidzein from Pueraria lobata(Wild.)Ohwi by coordination chromatography. The extract of Pueraria lobata(Wild.) Ohwi was mixed with Nickel sulfate and then purified by dry column chromatography in gradient systems composed of chloroform and methanol. The elution order of genistein and daidzein was opposite comparing with ordinary chromatography method, and the purity of genistein and daidzein were greater than 95%.

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Key concepts: Pueraria, Daidzein, Lobata, Genistein, Chemistry, Chromatography, Column chromatography, Elution