Proliferation inhibition induced by arsenic troxide on lung neoplasms cell line NCI H69

Purpose:To observe the effect of proliferation inhibition of Arsenic Trioxide(AS 2O 3) on lung cancer cell line NCI H69.Methods:Lung cancer cell line NCI H69 was treated with different concentrations of AS 2O 3. MTT assay was used to observe the proliferation status. Flow cytometry was used to measure the percentage of proliferating cell nuclear antigen (PCNA) positive cell. Results:AS 2O 3 could significantly inhibit the growth of NCI H69 cell, this effect was dose and time dependent. The percentage of PCNA positive cell was decreased gradually when concentration of AS 2O 3 was increased. Conclusions:Arsenic Trioxide could inhibit the proliferation activity of lung cancer cell line SW620. Reduction of PCNA which was related to the regulation of DNA synthesis and cell proliferation may be the mechanism of this proliferation inhibition.