Dynamic monitoring of serum inflammatory cytokines and products of oxidative stress in patients with acute intracerebral hemorrhape

Objective To monitor the serum levels of inflammatory cytokines and products of oxidative stress in patients with acute cerebral hemorrhage( AICH),and discuss the relationship of inflammation and oxidative stress in the pathogenesis of related complication of AICH. Methods 80 cases of patients with AICH were involved in the study and their cubital vein blood of 24 h after admission,25 ~ 48 h,49 ~ 72 h,7d,and 14 d were taken. Serum inflammatory factors IL-6 and TNF-α were tested using double antibody sandwich enzyme-linked immunosorbent assay( ELISA),and serum levels of products of oxidative stress,superoxide dismutase( SOD) and malondialdehyde( MDA) were measured with xanthine oxidase and thiobarbituric acid methods. All of the results were compared to that of normal people. Results Serum levels of IL-6,NF-α and MDA were increased significantly within 24 h and peaked within 49 ~ 72 h,and began to decrease at day 7. At day 14,serum IL-6,NF-α and MDA were all almost returned to the normal. While serum SOD level began to decline within 24 h and reached its lowest value within49 ~ 72 h and began to increase at day 7,returning to its normal value at day 14,without significant difference compared to control group. Conclusion The results suggested that the inflammatory cytokines,oxidative stress may have a function in the development of brain damage after intracerebral hemorrhage.