Effects of Injury-induced Stress on Serum TNF-α、IL-6、NO and Oxidative Stress in Diabetes Rats

Objective:To investigate the effect of injury-induced stress on serum TNF-α、IL-6、NO and enzymes of oxidative stress in diabetic rats.Methods: Diabetic rat model was induced by streptozocin and amputation of left lower limb was performed as severe traumatic stress.Serum TNF-α、IL-6、NO and anti-oxidant enzymic activities were evaluated before stress (injury-induced) and 2 h、 6 h、 12 h、 24 h、 72 h after stress respectively.Results: The level of glucose was significantly increased after stress.The level of TNF-α、IL-6、NO were significantly increased,and the peaks of TNF-α、NO were at 12 h after stress,the peak of IL-6 was at 6 h after stress.The level of SOD、GSH were significantly decreased and MDA imcreased after stress.The highest/lowest levels of SOD、GSH、MDA were at 12 h after stress. Conclusion: Injury-induced stress increases the level of TNF-α、IL-6、NO and reduces the antioxygenic ability in diabetes rats,and further impairs glucose regulation.