Analysis of genetic diversity of paradisefish Macropodus opercularis using RAPD markers

Random amplified polymorphic DNA(RAPD) technique was employed to detect the genetic variations in paradisefish Macropodus opercularis populations distributed in five geographical colonies.In this study,11 effective primers out of 40 random primers were selected to amplify genomic DNA,resulting in 139 repeatable RAPD bands.The 5 populations were shown to have polymorphism frequency of 26.44%-40.95%,Shannon genetic diversity index of 0.1042-0.1457 and Nei genetic diversity of 0.1543-0.2176.On the average,all the population showed the frequency of polymorphism of 84.89%,Nei genetic diversity of 0.3110 and Shannon genetic diversity index of 0.4606,indicating that the genetic variation of the paradiselfish was not from the intra-populations but from inter-populations.