Protective effect of ethyl pyruvate on renal injury in severe acute pancreatitis rats

Objective To explore the therapeutic role and its mechanism of ethyl pyruvate on renal injury induced by severe acute pancreatitis(SAP) in rats.Methods Seventy-two SD rats were randomly divided into three groups: sham operation group(S group),SAP group(P group) and ethyl pyruvate treatment group(T group).SAP model was established by retrograde infusion of 50 g/L sodium taurocholate into the biliopancreatic duct of rats.The rats were killed at 3 h,6 h and 12 h after operation.The serum level of TNF-α,IL-6,BUN,Cr and HMGB-1 were measured.The expression of NF-κB was detected by immunohistochemistry.The renal tissues were taken for HE staining and electron microscopic examination.Results In group P,serum levels of TNF-α,IL-6,BUN,Cr and expression of NF-κB in the renal tissues were higher than those in group S(P0.05),HMGB-1 levels were higher than those in group S at 6 h and 12 h(P0.05).The serum level of TNF-α,IL-6,BUN,Cr,HMGB-1 and expression of NF-κB in the renal tissues in T group were lower than those in group P(P0.05).Renal pathological damage was milder in group T than those in group P under microscopy and electron microscopy.Conclusion Ethyl pyruvate plays a protective role against renal injury in severe acute pancreatitis rats.The underlying mechanism may be related to inhibition of TNF-α,IL-6,HMGB-1 and suppression of NF-κB.