Protective Effect of Yulangsan Flavonoids on Acute Liver Injury Induced by Carbon Tetrachloride in Mice

Objective:To observe the protective effect of Yulangsan flavonoids(YLSF) on acute liver injury induced by carbon tetrachloride in mice.Method: The Kunming mice were divided into normal control group(NC),model group(MC),high-,medium-and low-doses of YLSF groups,and biphenyldicarboxylate group(BPDC).The mice of NC and MC groups were treated with normal saline(NS),and all doses of YLSF groups and the positive control group were treated with YLSF(112,56,28 mg·kg-1) and BPDC(150 mg·kg-1) respectively.All mice were treated with drugs or NS for 7 consecutive days ig,qd.One hour after the last administration of drugs,each group of mice was induced by 0.1% CCl4,ip,except the NC group.The activities of aspartate aninotransfer as(AST),alanine aminotransferase(ALT) and Alkaline phosphatase(ALP),the content of Albumin(Alb),the ability of Total antioxidant capacity(T-AOC) in serum,the content of Malondialdehyde(MDA),and the activity of Superoxide dismutase(SOD) in liver tissue were investigated by spectrophotometry,and the degree of hepatic injury was examined.Result: In low-,medium-and high doses of the YLFS group,the activity of AST,ALT,Alb,ALP and the level of T-AOC in the serum were AST(40.49±10.41),(32.97±2.58),(27.57±7.98) U · L-1,ALT(129.69±18.02),(90.79±7.02),(66.75±20.90)U · L-1,Alb(4.53±1.05),(5.15±1.62),(5.68±0.72) g · L-1,ALP(40.06±1.47),(35.77±2.66),(32.87±4.19)U · L-1 and T-AOC(4.33±1.56),(6.49±0.96),(8.15±0.76)U · mL-1,the activity of SOD and the content of MDA in the tissue were SOD(122.01±14.47),(165.49±18.25),(191.66±37.95) U · mg-1 and MDA(15.07±1.75),(12.27±1.79),(9.65±1.76) nmol·mg-1 respectively.The activities of AST,ALT and ALP in serum(P0.01) and the content of MDA in liver(P0.01) were decreased,the content of Alb and the activity of T-AOC in serum(P0.01),and the activity of T-SOD in liver(P0.01) were enhanced obviously by treatment with medium-and high-doses of YLSF.Then in low does of YLSF group,the activities of AST and ALP in serum(P0.01,P0.05),and the content of MDA in liver(P0.01) were decreased,and the content of Alb in serum(P0.01) was increased significantly,but the activities of ALT in serum and T-SOD in liver,and level of T-AOC in serum could not be significantly affected.The degree of hepatic injury in all doses of YLSF groups could be lessened.Conclusion: YLSF has protective effect on acute liver injury induced by CCl4 in mice.The mechanism may be involved in attenuating free radical and inhibiting the lipid peroxidation.