Culture and osteogenic differentiation of adipose-derived stem cells in Sprague-Dawley rat

Objective:To study the method of isolating and culturing stem cells from rat adipose tissue andto determine the osteogenic capacity of adipose- derived stromal cells(ADSCs) harvest from rat in vitro.Method:ADSCs isolated from rat inguinal fat pads were digested with collagenase.After primary culture incontrol medium and expansion to the 2nd passages,the cells were incubated in induced medium for 2￣4weeks to induce osteogenesis.Osteogenic differentiation was tested by alkaline phosphatase and Von Kossastaining.Result:ADSCs could be isolated from rat adipose tissue,after osteogenesis induction,the result foralkaline phosphatase and Von Kossa staining was positive.Conclusion:Adipose- derived stromal cells can beisolated from rat adipose tissue,their biological characteristics are analogy to mesenchymal stromal cells(MSCs),and have the potential to differentiate into osteogenic lineage.It may be a source for tissueengineering.