Tissue array-based immunohistochemical analysis of PSA,P504s,CK34βE12,P63 and Ki-67 in prostate cancer

【Objective】Using tissue array-based immunohistochemistry to assess the expression levels of PSA,P504s,CK34βE12,P63and Ki-67 in the pathological diagnosis of prostate cancer.【Methods】A tissue array containing 24 cases of various prostate diseases(14 cases of adenocarcinoma,8 cases of hyperplasia,1 case of leiomyoma and 1 case of metastatic carcinoma) was analyzed immunohistochemically. The expression levels of the selected markers in tissues were recorded semi-quantitatively,and compared between hyperplastic and cancerous prostate groups.【Results】All the 8(100%) herperlastic cases showed strong staining for PSA,while only 1/14(7%) of adenocarcinoma cases was strongly PSA positive(P=0.0001). The 8 hyperplastic prostate cases were also positive for CK34βE12 and P63,but all the 14 prostate cancer cases were negative for these two markers(P0.05). In contrast,P504s was positive in all the 14(100%) cases of the prostate adenocarcinoma,whereas only 1/8(12.5%) of the hyperplastic prostates showed staining for P504s(P0.05). Eight(57.1%) of the 14 prostate cancers showed a high expression level of Ki67,but none of the eight hyperplastic prostates was positive for Ki67(P﹦0.0264).【Conclusion】 Our results indicate that combined immunohistochemical examination of PSA,P504s,CK34βE12,P63 and Ki-67 has significant value in the pathological diagnosis of prostate specimens. A positive result for P504s can assist the diagnosis of prostate cancer,while a staining for CK34βE12 and P63 may help to eliminate cancerous changes of a prostate. Tissue array technology allows high-throughput,economic and precise immunohistochemical analyses of tissue samples,and has a great value in pathological studies and diagnostic assessments.