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2003•Zhongguo kangganran hualiao zazhiRequires access

Study of an integron mediated VIM-2 type metallo β-lactamase

Han Li-zhong

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Abstract

Objective: To analyze the resistant mechanism to imipenem in a strain of Pseudomonas aeruginosa . Methods:Using modified three dimension test to analyze and classify the β lactamase produced by the strain; detecting the resistance genes by PCR; to screen and classify the integron by PCR RFLP; to amplify the variable region of integron by long fragment PCR; the PCR products were sequenced subsequently. Results:Clinical strain of Pseudomonas aeruginosa RJ213 carrying an integron mediated vim 2 β lactamase was found. Conclusions:Integron mediated VIM 2 type metallo β lactamase exists in Shanghai, clinical isolate which cause resistance to imipenem.

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What this paper is about

Objective: To analyze the resistant mechanism to imipenem in a strain of Pseudomonas aeruginosa . Methods:Using modified three dimension test to analyze and classify the β lactamase produced by the strain; detecting the resistance genes by PCR; to screen and classify the integron by PCR RFLP; to amplify the variable region of integron by long fragment PCR; the PCR products were sequenced subsequently. Results:Clinical strain of Pseudomonas aeruginosa RJ213 carrying an integron mediated vim 2 β lactamase was found. Conclusions:Integron mediated VIM 2 type metallo β lactamase exists in Shanghai, clinical isolate which cause resistance to imipenem.

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Available abstract

Objective: To analyze the resistant mechanism to imipenem in a strain of Pseudomonas aeruginosa . Methods:Using modified three dimension test to analyze and classify the β lactamase produced by the strain; detecting the resistance genes by PCR; to screen and classify the integron by PCR RFLP; to amplify the variable region of integron by long fragment PCR; the PCR products were sequenced subsequently. Results:Clinical strain of Pseudomonas aeruginosa RJ213 carrying an integron mediated vim 2 β lactamase was found. Conclusions:Integron mediated VIM 2 type metallo β lactamase exists in Shanghai, clinical isolate which cause resistance to imipenem.

Key concepts: Integron, Pseudomonas aeruginosa, Imipenem, Microbiology, Strain (injury), Biology, Bacteria, Antibiotic resistance

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