Development of Pneumococcal capsular polysaccharide type 14-tetanus toxoid conjugate vaccines

Objective To identify the reactive conditions for preparing PNCPS protein conjugates in order to cellect experiences in the development of conjugate vaccines afterwards. Methods 14 TT conjugates were prepared by carbodiimide mediated coupling of PNCPS with tetanus toxoid(TT). Female NIH mice were immunized with conjugates or pure PNCPS type 14, and the PNCPS antibodies in the sera of animals were detected by ELISA. Results The yield and composition of the conjugates tests showed that there are PNCPS and TT in conjugates. All of the conjugates elicited high level antibody response and induced immunogenic memory in mice, comparing to pure PNCPS. Conclusion 14 TT conjugates were successfully prepared with feasible technology.