Preparation and Immunogenicity of Pneomococcus Type 6B Capsular Po-lysaccharide - Tetanus Toxoid Conjugate Vaccine

Objective To prepare and study the immunogenicity of pneomococcus type 6B capsular polysaccharide (6B - PNCPS) - tetanus toxoid (TT) conjugate vaccine. Methods 6B - PNCPS - IT was activated by Cyangen Bromide (CNBr) and reacted with adipic acid dihydrazide (ADH), then 6B - PNCPS - TT conjugate was prepared by carbodiimide - mediated coupling of 6B-PNCPS with tetanus toxoid (TT) . NIH mice were immunized with 6B - PNCPS - TT conjugate, using pure 6B - PNCPS as control, and the PNCPS antibody titers in the sera of them were detected by ELISA. Results Gel chromatography proved that, compared with that of 6B - PNCPS, the relative molecular weight of 6B - PNCPS - TT was large. The ratio of polysaccharide to protein was 1.42- 1.66. The conjugate vaccine showed the serological characteristic of 6B -PNCPS, and the liters of specific IgG induced by it was significantly higher than that induced by 6B - PNCPS. Conclusion The prepared 6B - PNCPS - TT conjugate vaccine showed good immunogenicity.