Effects of Ketamine on Survival Rate and Serum Concentration of Tumor Necrosis Factor-α,Interleukin-6 in Septic Rats

Objective To observe the effects of ketamine on survival rate and serum concentration of tumor necrosis factor-α(TNF- α),interleukin-6(IL-6)in septic rats after ligature of cecum and to investigate the possibe mechanism.Methods 132 male SD rats were randomly allocated into 3 groups:①Normal control group,②Control group,③Ketamine group.Each group was again divided into 4 sub- group(Ⅰ,Ⅱ,Ⅲ,Ⅳ).A cecum ligation and puncture procedure was used to male septic rats model.After half an hour of opera- tion.Ketamine group was administed with ketamine 50 mg/kg every 12 hours imtramuscularly,whereas normal control group and control group were given equivalent volume normal saline until rats died or 5 days observation ended.The survival period was observed in each subgroups Ⅱ,Ⅲ,Ⅳ for measurement of TNF-α.IL-6 at 0 hour before operation,4 hours after operation and the dying point of each rat of control sub- group Ⅳ.Results Decrease in the levels of TNF-α and IL-6 were found at anytime after ketamine intervention(P0.01).The 5 days sur- vival rate:a significantly higher survival rate was found in ketamine group than in control group(P0.01).Conclusion Ketamine could restrain the TNF-α,IL-6 by the CLP(cecamhigature procedure)and rose the survival rate of rats with sepsis.