Study on the killing activity of DC-CIK cells pulsed with gastric cancer cells of heat shock against cancer cells

Objective To study the killing effect of dendritic cells(DC) and cytokine-induced killer(CIK) cells pulsed with specific antigens against gastric cancer cells.Methods Peripheral blood mononuclear cells(PBMC)were isolated from healthy subjects.DC and CIK cells were induced by different cytokines.Gastric cell line MKN-28 lysates of heat shock were used as antigen and pulsed DC,which co-cultured with CIK cells.The phenotypes of DC and CIK cell membranes were determined by flow cytometry,and the killing activity of CIK against MKN-28 was determined by(MTS).Results Compared with CIK,the co-cultured DC-CIK presented a significantly higher proliferation.The numbers of CD3+CD8+ and CD3+CD56+ cells increased,and the killing effect was enhanced.The cytotoxic activity to MKN-28 of DC-CIK pulsed with MKN-28 antigen(Ag-DC-CIK) group was(57.96±2.23)%,which was more strong than(38.02±2.06)% of DC-CIK pulsed without MKN-28 antigen group and(29.78±1.84)% of CIK group,respectively [the ratio of effect to target =20.0∶ 1].Conclusions Heat shock tumor-lysate-pulsed DC can strengthen the proliferation and killing activity of CIK against gastric cancer cells.