2014•Zhongguo shiyan fangjixue zazhiRequires access
Pharmacokinetics of Veratric Acid in Rats
AN Yan-na
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Abstract
Objective: To study pharmacokinetics of veratric acid in rats. Method: Veratric acid was administrate to rats by oral gavage,blood samples were collected at various time points,HPLC was adopted to determine the content of veratric acid in blood samples by taking 3,4-dihydroxybenzoic acid methyl ester as an internal standard,mobile phase of acetonitrile( A)-1% acetic acid( B)( 0-11 min,5%-57% A; 11-13 min, 57%-5% A), detection wavelength was set at 256 nm, plasma concentration-time curve was drawn, pharmacokinetic parameters were calculated by Kinetica 4. 4 software. Result: After oral gavage administration, veratric acid existed secondary peak phenmenon in rats,T max were( 45. 00 ± 10. 00) and( 31. 00 ± 8. 22) min, respectively; C max of high and low dose groups were( 176. 85 ± 28. 89) and( 68. 14 ± 12. 58) mg·L- 1,t 1 /2 were( 87. 74 ±20. 74) and( 227. 18 ± 93. 78) min,AUC 0-∞ were( 50 363. 50 ± 11 667. 7) and( 18 830. 32 ± 4 354. 35) min ·mg ·L- 1,MRT 0-∞ were( 201. 39 ± 16. 72) and( 227. 40 ± 21. 37) min, respectively. Conclusion: Veratric acid had a long in vivo acting time due to its quick absorption and slow elimination.