HPLC determination of senkyunolideI in mice plasma and its pharmacokinetics

Objective:To establish an HPLC method for the determination concentration of senkyunolideⅠin mice plasma for pharmacokinetic study.Methods:The mice were intravenously administrated of a single dose or intragastricly administrated senkyunolide I(104 mg·kg-1).The plasma samples after protein settlement processing were analyzed for the senkyunolideⅠ concentration by HPLC.The Megres-C18(150 mm×4.6 mm,5 μm)column was adopted,the mobile phase was acetonitrile-0.2% glacial acetic acid(1:3,v/v)at a flow rate of 1.0 mL·min-1 under 30 ℃,the senkyunolide H was marked as the internal standard,and the detection wavelength was set at 278 nm.Results:Excellent liner relationship was obtained in the range of 0.075 to 30 mg·L-1,the limit determination of senkyunolideⅠwas 0.075 mg·L-1.The recovery of the method was more than 85%,and the intra-and inter-day RSDs were less than 5%.The samples were stable for 24 h at room temperature,5 days at-4 ℃,and remained stable after three freeze-thaw cycles.The pharmacokinetic parameters were as follows:t1/2 31.93 min,AUC(0-∞) 2738.80 mg·min·L-1,MRT(0-∞) 31.61 min in mice with intravenous administration,and t1/2 40.07 min,AUC(0-∞) 892.42 mg·min·L-1,MRT(0-∞) 61.38 min in mice with intragastric administration.The absolute bioavailability of senkyunolideⅠ in mice was calculated to be 32.19%.Conclusion:The method is reliable,simple,rapid,and suitable for the pharmacokinetic study and determination of senkyunolideⅠ in plasma.