Effect of FTY720 on in vitro MCF-7 proliferation and apoptosis reaction

Objective To study effect of FTY720 on in vitro MCF-7 proliferation and apoptosis.Methods MCF-7 cells were cultured in vitro and different concentrations of FTY720 were added to its culture medium,continued to culture for 48 hours,determine MCF-7 cell proliferation by MTT assay;observe cell modality by Switzerland-Giemsa staining;determine cell cycle and apoptosis rate by Flow cytometry Detection.Results FTY720 at concentration of 6 400 ng/ml could significantly suppress in vitro MCF-7 proliferation(P0.05)with the inhibition rate of 62.0% and in a concentration-dependent manner,cells appeared apoptotic modality,MCF-7 cells were arrested in G1 phase and promote their apoptosis and the apoptotic rate in a concentration dependence.Conclusion Adding a certain concentration of FTY720 to In vitro MCF-7 cell culture medium significantly inhibits the cell proliferation,regulats the cell cycle and induces their apoptosis.