2009•Anhui nongye kexueRequires access

Study on the Genetic Variation of Dendrobium by RAPD Molecular Markers

Luo Yuanhua

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Abstract

[Objective] The aim of research was to detect the genetic variation of Dendrobium by RAPD molecular marker.[Method] With 4 different varieties of Dendrobium and 1 variety in vitro as materials,after DNA extraction,10 bp random primers was used for RAPD detection of genetic variation.[Result]Burana Greenand Pink Thamond 2 varieties not found genetic variation;the genetic variation rate of BigPanda was 3.3%,the genetic variation rate of Thailand White was 23.3%;the genetic variation rate of Red Sonia in vitro was 10.9%.[Conservation] RAPD detection technology of genetic variation for Dendrobium was established.

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What this paper is about

[Objective] The aim of research was to detect the genetic variation of Dendrobium by RAPD molecular marker.[Method] With 4 different varieties of Dendrobium and 1 variety in vitro as materials,after DNA extraction,10 bp random primers was used for RAPD detection of genetic variation.[Result]Burana Greenand Pink Thamond 2 varieties not found genetic variation;the genetic variation rate of BigPanda was 3.3%,the genetic variation rate of Thailand White was 23.3%;the genetic variation rate of Red Sonia in vitro was 10.9%.[Conservation] RAPD detection technology of genetic variation for Dendrobium was established.

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Available abstract

[Objective] The aim of research was to detect the genetic variation of Dendrobium by RAPD molecular marker.[Method] With 4 different varieties of Dendrobium and 1 variety in vitro as materials,after DNA extraction,10 bp random primers was used for RAPD detection of genetic variation.[Result]Burana Greenand Pink Thamond 2 varieties not found genetic variation;the genetic variation rate of BigPanda was 3.3%,the genetic variation rate of Thailand White was 23.3%;the genetic variation rate of Red Sonia in vitro was 10.9%.[Conservation] RAPD detection technology of genetic variation for Dendrobium was established.

Key concepts: RAPD, Dendrobium, Genetic variation, Biology, Genetic marker, Genetic variability, Genetics, Botany

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