2009•Xinjiang nongye kexueRequires access

Cloning and Vector Construction of N,P,G and L Gene from Rabies Virus Oral Vaccine Strain SRV_9

Jingjing Zhu

Abstract

Using the total RNA isolated from rabies virus as a template,the cDNA encoding N,P,G and L was amplified by reverse transcription polymerase chain reaction method.Further,the PCR product was cloned into pGEM-T Easy and sequenced.The cDNA was verified identicaly with related literatures by sequenced,and the recombinant vectors are constructed successfully.

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Key concepts: Rabies virus, Complementary DNA, Virology, Cloning (programming), Biology, Recombinant DNA, Vector (molecular biology), Reverse transcription polymerase chain reaction