Structure of Full-length Plasmid of Rabies Virus Oral Vaccine Strain SRV_9

Rabies Virus is a lethal zoonosis.To deeply study genomic characterization of SRV9 and construct the new vector,several pairs of primers was designed according to the full-length genomic sequence of rabies virus oral vaccine strain SRV9(GenBank Accession No AF499686).Using the isolated total RNA from rabies virus as a template,five DNA fragments covering the entire genome of SRV9 were amplified by RT-PCR,and inserted into pMDI8-T or pGEM-T vector separately and sequenced.Fragments were all cloned into pBluescript SK(+) vector thereafter sequencing correctly.Five fragments were ligated by using amplified fragment containing themselves' restriction enzyme sites to obtain the full-length plasmid clone pBLSRV9 of SRV9.Compared with the genome sequences of homology analysis,SRV9 have the stable hereditary trait.Therefore the construction of full-length plasmid provides foundation to deeply study of rabies virus on the copy,infectious,pathogenicity and the mechanism of molecular immunity and develop the new vaccine.