Immunohistochenistry study on Nogo-A expressions in mice after spinal cord injury

Objective To investigate the Nogo-A expressions in mice after spinal cord injury.Methods C57 mice were divided into spinal cord injury group,sham group and control group.Samples were acquired at different time points(1 h,12 h and 24 h after injury) in each group.Immunohistochemistry was performed in each group.Results Nogo-A was obviously expressed in pericaryon and neuron fibers which had neurite formation.The number of positive cells of Nogo-A and intensity of immunoreactions were increased as the survival time prolonged after injury.Conclusion The expressions of Nogo-A were increased within 24 h after spinal cord injury,which will induce difficulty in nerve regeneration.