The Changes of Nogo-A Expression in the Neurons of Acute Complete SCI Rats after GAP-43 Therapy

Objective To provide evidence for the therapy effect of neuronal factor GAP-43 after spinal cord injury by observating the expression changes of Nogo-A in complete spinal cord injury rats.Methods Seventy-five 8-week-aged female rats were divided into three groups randomly:the GAP-43 antibody therapy group,the GAP-43 antigen therapy group and control group,with 25 rats in each group.After GAP-43 antibody or antigen being injected into the sectioned sites of the damaged spinal cord,the behavioral scores of the rats were evaluated at different time,while H-E staining and immunohistochemical staining were used to examine the expression of Nogo-A in the damaged spinal areas.Results The results showed that the BBB score was the lowest in the control group and the highest in the antigen group,in which remarkable recovery of pathological changes of spinal cord was observed.The positive neurons'amount of Nogo-A was fewer than other groups and had negative correlation with the physical reconstruction.Conclusion GAP-43 has an antagonism with Nogo-A and has the potent neuro protective effects in the therapy for SCI.