The effect of N-acetylcysteine on expression of nuclear factor-κB on hepatic ischemia infury in rat models

Objective To explore the role of tetramethylpyrazine on the expression of nuclear factor-κB(NF-κB)of rats with hepatic ischemia-reperfusion(I/R)injury,and study the possible mechanism of hepatic protection affected by TMP against acute I/R injury.Methods Thirty male Wistar rats were divided into three groups in randomly and average.group a for sham operation;group b for ischemia-reperfusion group c injected with N-acetylcysteine before I/R.3 hours later the rats were killed and the serum was taken to detect the ALT,bilirubin,The expression of NF-κB in heptic tissues was detected by immunohistochemical method.Results The expression of NF-κB and the level of ALT,bilirubin,PMN increased markedly during heptic ischemia-reperfusion compared to sham group(P0.01);decreased significantly after administration of tetramethylpyrazine compared to I/R group(P0.01).Conclusion Tetramethylpyrazine can protect heptic from acute I/R injury,partly through inhibiting activation of NF-κB and PMN to alleviate inflammation.