Effect of Oxymatrine on Expression of c-myc,PSMD9 and CDK4 mRNA in Human Colon Carcinoma LoVo Cells

Objective:To explore the molecular mechanism of inhibiting colon cancer cell strein LoVo proliferation and inducing apoptosis by oxymatrine(OM) Method:Flow cytometry was used to detect the LoVo cells apoptosis and cell cycle distribution.Fluorescence quantitative PCR was used to detect cell proliferation-related genes like the c-myc,proteasome modulator 9(PSMD9),CDK4 gene expression when LoVo was treated with 0.25,0.5 g·L-1OM.Result:OM had no significant effect on apoptosis in colon cancer LoVo cells when the treatment of OM lasted 48 h and the concentration was lower than 0.5,0.25 g·L-1 OM can inhibit c-myc gene expression in LoVo when duration of action last 24 h(P0.05).When the dose increated to 0.5 g·L-1 and duration of action was 48 h,OM could inhibit c-myc,CDK4 gene expression in LoVo cells(P0.01,P0.01).When duration of action was extended to 96 h,0.5 g·L-1 OM could inhibit the c-myc,PSMD9,CDK4 gene expression in LoVo cells(P0.05,P0.01,P0.01).Conclusion:OM at Lower dose could significantly inhibit the proliferation of human colon cancer LoVo cells,the mechanism may be related to reducing c-myc,PSMD9,CDK4 expression in LoVo cells.