Extraction,Purification and Content Determination of Polysaccharide in Gingko Leaves

Objective The extraction and purification methods for polysaccharide in Gingko leaves were studied and the content of polysaccharide in it was determined.Methods The condition of each factor for extraction and pruification from the leaves of Gingko was established through the experiments of extraction times, different ratios of material to extraction solvent and extraction time.The method of phenol-sulfuric acid was applied to determination of the content of the polysaccharide.Results The conditions of the factors were extraction temperature 80℃,extraction time 3h,material to extraction solvent 1:30 ,extraction 2 times and activated carbon for purification.Raw polysaccharide in gingko leaves was 12.2%,the polysaccharide after prurified was 9.8%, the content of polysaccharide in the raw polysaccharide was 35.67%,and in the pure one was 43.32%,Conclusion The purification method is efficient and stable. The content of polysaccharide in gingko leaves is 4.3%.