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2013•Strait Pharmaceutical JournalRequires access

Determination of Emodin and Chrysophanol in Jianweixiaoyan Particles by HPLC

Ye Hu

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Abstract

OBJECTIVE To establish an HPLC method for the determination of Emodin and Chrysophanol in Jianweixiaoyan. METHODS The Symmetry C18column of 4. 6mm × 150mm,3. 5μm was used. The mobile phase consisted of methanol-0. 1% H3PO4( 85∶15). The detective wavelength was 254nm. RESULTS The Linearity of this method was well with the sugar-free type average recovery of 99. 87% and the sugar tupe average recovery of99. 94%. CONCLUSION The method is simple and accurate. It can be used for the determination of the contents of Emodin and Chrysophanol in Jianweixiaoyan Particles.

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What this paper is about

OBJECTIVE To establish an HPLC method for the determination of Emodin and Chrysophanol in Jianweixiaoyan. METHODS The Symmetry C18column of 4. 6mm × 150mm,3. 5μm was used. The mobile phase consisted of methanol-0. 1% H3PO4( 85∶15). The detective wavelength was 254nm. RESULTS The Linearity of this method was well with the sugar-free type average recovery of 99. 87% and the sugar tupe average recovery of99. 94%. CONCLUSION The method is simple and accurate. It can be used for the determination of the contents of Emodin and Chrysophanol in Jianweixiaoyan Particles.

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Available abstract

OBJECTIVE To establish an HPLC method for the determination of Emodin and Chrysophanol in Jianweixiaoyan. METHODS The Symmetry C18column of 4. 6mm × 150mm,3. 5μm was used. The mobile phase consisted of methanol-0. 1% H3PO4( 85∶15). The detective wavelength was 254nm. RESULTS The Linearity of this method was well with the sugar-free type average recovery of 99. 87% and the sugar tupe average recovery of99. 94%. CONCLUSION The method is simple and accurate. It can be used for the determination of the contents of Emodin and Chrysophanol in Jianweixiaoyan Particles.

Key concepts: Emodin, Chromatography, Chemistry, High-performance liquid chromatography, Methanol, Organic chemistry

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