Effects of arctigenin on PCNA expression and cell cycling in human Human Esophageal Cancer Cells

Objective To investigate the effects of ARG on PCNA expression and cell cycling in invitro cultured esophageal cancer-1 cells Methods EC-1 cells were treated with different concentration ARG.Proliferation of EC-1 cell inhibited by ARG were assessed by MTT.The cell cycle was measured using flow cytometry(FCM),and the level of proliferating cell nuclear antigen(PCNA)with immunohistochemistry(IHC).Results ARG could inhibit the growth and significantly suppressed expression of PCNA of EC-1 cells in a dose/time dependent manner(P0.05).G0/G1 to S phase transition was blocked after treated with ARG,the percentage of G1 phase of the cell cycle was significantly increased,whereas the percentage of S phase was remarkably decreased.Conclusion The findings suggested the ARG could inhibit the growth and expression of PCNA,and retarded cell cycle of EC-1.