Effect of Decoction of Cooling Blood on Hacat Cell Apoptosis in Vitro

Objective To investigate the decoction of cooling blood on HaCaT cell growth and apoptosis.Methods The effect of decoction of cooling blood on the growth of HaCaT through MTT assay,the apoptosis of HaCaT by combined Annexin-V protein iodide staining,the cell cycle of HaCaT were studied by a propidium iodide method.Results Decoction of cooling blood inhibited the HaCaT cells in a time-manner,with 0.4～10mg/ mL.After 48h induced apoptosis of HaCaT cells,and the apoptosis rate reached(31.77±5.88 )%at concentration of 10mg/mL.Flow cytometric analyses showed that HaCaT cells treated with decoction of cooling blood were accumulated in G_1 phase in the cell cycle,the ratio of cells in and S phase was decreased at 48h treatment. Conclusion Decoction of cooling blood is able to inhibit the proliferation of HaCaT cells by regulating the cell cycle and inducing the cell apoptosis.