Study on the Moiphogenesis Way of Acanthopanax senticosus

Using Acanthopanax senticosus calli as materials,the regeneration plants through organogenesis and somatic embryogenesis were got.The results showed that the transplanting survival rate was 47%(organogenesis) and 86% (somatic embryogenesis),respectively.Best combination for adventitious bud was MS medium supplemented with 0.2 mg/L NAA and 3.0 mg/L BA;while for somatic embryogenesis,MS medium supplemented with 20 g/L sucrose, 20 g/L glucose without hormonal was better than others.Histological observation showed that buds mostly formed on the surface of the callus,and somatic embryogenesis was similar to the process of zygotic embryogenesis,and it could be induced from internal callus and originated single cell.