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2011•Liaoning Forestry Science and TechnologyRequires access

Research on somatic embryogenesis and seedling formation regulation of Acanthopanax senticosus

Zheng Lü

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Abstract

The effect of plant growth regulating substances on embryonic callus induction,somatic embryogenesis and rootting were investigated by means of vitro culture from leaf of test-tube seedlings of Acanthopanax senticosus.The results showed that the optimum medium on embryogenic callus induction was MS+6-BA 1.5 mg·L-1+2,4-D 1.0 mg·L-1.The optimum medium on growth and development of somatic embryo was MS+6-BA 1.0 mg·L-1+IBA 0.5 mg·L-1.The optimum medium on rootting was1/2MS+NAA 0.4 mg·L-1.

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What this paper is about

The effect of plant growth regulating substances on embryonic callus induction,somatic embryogenesis and rootting were investigated by means of vitro culture from leaf of test-tube seedlings of Acanthopanax senticosus.The results showed that the optimum medium on embryogenic callus induction was MS+6-BA 1.5 mg·L-1+2,4-D 1.0 mg·L-1.The optimum medium on growth and development of somatic embryo was MS+6-BA 1.0 mg·L-1+IBA 0.5 mg·L-1.The optimum medium on rootting was1/2MS+NAA 0.4 mg·L-1.

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Available abstract

The effect of plant growth regulating substances on embryonic callus induction,somatic embryogenesis and rootting were investigated by means of vitro culture from leaf of test-tube seedlings of Acanthopanax senticosus.The results showed that the optimum medium on embryogenic callus induction was MS+6-BA 1.5 mg·L-1+2,4-D 1.0 mg·L-1.The optimum medium on growth and development of somatic embryo was MS+6-BA 1.0 mg·L-1+IBA 0.5 mg·L-1.The optimum medium on rootting was1/2MS+NAA 0.4 mg·L-1.

Key concepts: Somatic embryogenesis, Callus, Seedling, Embryogenesis, Biology, Embryo, Botany, Somatic cell

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