Anti-tumoral effect of Trichinella spirialis on Hepa 1-6 hepatoma carcinoma cell in the C57BL/6 mice.

Objective To observe the anti-tumer effect of Trichinella spirialis on Hepa1-6 hepatoma carcinoma cell.Methods The C57BL/6 mice were randomly divided into 8 groups with 10 mice in each group.The mice of Group 1,5,Group 2,6 and Group 3,7 were infected with non-attenuated T.spirialis,60Co-attenuated T.spirialis and ultraviolet-attenuated T.spirialis,respectively,but Group 4,8 were not infected by T.spirialis as control.The Group 1,2,3,4 and Group 5,6,7,8 were inoculated with Hepa1-6 hepatoma carcinoma cells at the 7 d befor and the 11 d after infected with T.spirialis.The different group mice were dissected at the 25 d after inoculated with Hepa1-6 hepatoma carcinoma cells.The anti-tumer effects of T.spirialis was observed by determining the size and weight of tumors,the number of CD3+,CD4+,CD8+,CD4+/CD8+ and CD4+/CD3+ of the mice.Results The size and weight of the tumors in the mice which were infected by T.spirialis,60Co-attenuated T.spirialis and ultraviolet-attenuated T.spirialis were smaller than the control group(P0.05).The percentage of CD3+,CD4+,CD4+/CD8+ and CD4+/CD3+ in test groups were higher than the control group(P0.01 or P0.05).Conclusion The results showed that T.spirialis that attenuated by different methods and non-attenuated T.spirialis could inhibit the growth of Hepa1-6 hepatoma in vivo,in which the antitumor function of non-attenuated T.spirialis is better than the other test groups.