Expression and role of SCAP in models of cultured steatosis hepatocytes

Objective To explore the expression and role of SCAP in the models of cultured steatosis hepatocytes.Methods Steatosis models of hepatocytes was established by adding oleic acid to the growing L-02 cell strain.The expression of SCAP mRNA was measured with RT-PCR and the protein expression of SCAP were measured by Western blot,lipid droplets in the hepatocytes were observed with oil red staining and the contents of triglyceride in hepatocytes were measured with analyzed kit.Results In model groups,mRNA expression of SCAP increased at 24h,and elevated remarkably at 72h compared with the normal control group(P0.01),and protein expression of SCAP increased at 24h,and elevated remarkably at 120h compared with the control group(P0.01).Triglyceride contents in hepatocytes of these groups also increased.Through oil red staining,a few lipid droplets were observed at 24h and steatosis hepatocyte increased greatly at 120h.Conclusion The overexpression of SCAP in model groups could lead to disturbance of lipid metabolism and probably SCAP participates the process of steatosis of hepatocytes.