Effect of rosiglitazone on proliferation of vascular smooth muscle cell induced by high glucose

Objective: To investigate the effect of Rosiglitazone(RSG) on the proliferation of rat aortic vascular smooth muscle cells(VSMCs) induced by high glucose and the possible mechanism involved in the effect.Methods: The smooth muscle cells were cultured from the thoracic aorta of Sprague-Dawley(SD) rat.The viability of VSMCs was determined by3-(4,5-dimethy 1-2-thiazoly) 2,5-dipheny1-2H-tetyazolium bromide(MTT) assay.The cells cycle was examined by flow cytometry.The protein expression of proliferating cell nuclear antigen(PCNA) in VSMCs was evaluated by Western blotting.The mRAN expression of Matrix metalloproteinases-2(MMP-2) of VSMC was determined by RT-PCR.Results: Rosiglitazone at the concentration of 10μmol/l remarkedly inhibited the viability of VSMCs(P0.01),the expression of PCNA(P0.05)and the level of MMP-2 mRNA(P0.01) in VSMCs.Meanwhile,RSG decreased the proportion of VSMC S periods(P0.01) but increased the proportion of VSMCs G0/G1 periods(P0.01).Conclusion: These findings suggest that RSG has its inhibiting effect on high glucose-induced VSMCs proliferation,at least partly by the way of halting the cell cycles and inhibiting PCNA expression and MMP-2 synthesis.