Study on the Quality Difference of NILs with HMW-GS 17+18 and 7+8 in Longmai 20

In order to determine the genetic differences between high molecular weigh glutenin subunits(HMW-GS) 17+18 and 7+8,HMW-GS 17+18 was introduced into Longmai 20 by 6 consecutive backcrosses with biochemical marker assisted selection.The near isogenic lines(NILs) of HMW-GS 17+18 and 7+8 were obtained and grown in the experimental field of Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences in 2006.The field experiments were designed using the method of two line-contrast arrangement with four replicates.The result of the NILs showed as below,as compared to NILs with subunit 7+8,the NILs with subunit 17+18 were higher in gluten index,Zeleny sedimentation,development time,stability,breakdown time,respectively.The impact of the HMW-GS 17+18 on gluten strength was positive in NILs containing HMW-GS 5+10,suggesting that HMW-GS 17+18 could be a good subunit for breeding strong gluten wheat.