Study on the Quality of NILs of Wheat Cultivar Longfumai 3 with HMW-GS Null and 1 Subunits

【Objective】 The study was conducted to determine the genetic differences in high molecular weight glutenin subunits(HMW-GS)Null and 1.【Method】HMW-GS 1 was introduced into Longfumai 3 by five consecutive backcrosses with biochemical marker assisted selection.The near isogenic lines(NILs)of HMW-GS Null and 1 were obtained and grown the of Crop Breeding Institute of Heilongjiang Academy of Agricultural Science experimental fields in 2004 and 2005.The field experiments were designed using a two line-contrast arrangement with four replicates.【Result】 The two-year average results showed that the statistical differences in quality parameters between Longfumai 3 with 1 subunit and with Null were not significant in flour protein content,dry gluten content,water absorption.However the gluten index,Zeleny sedimentation,the ratio of sedimentation/dry gluten,development time,stability and breakdown time in the NIL with 1 subunit were increased by 5.8%,9.3%,8.6%,127.3%,79.2%,53.6%,respectively.This was in comparision to those in the NIL with Null.The ratio of wet gluten/dry gluten and degree of softening were decreased by 1.7%,16.5%,respectively.【Conclusion】The impact of the HMW-GS 1 on gluten strength was highly positive in NILs containing HMW-GS 5+10,suggesting that HMW-GS 1 could be a necessary subunit for breeding strong gluten wheat.