Effect of ligustrazine on gentamicin ototoxicity-induced expression of heat shock protein 70 mRNA in stria vascularis of guinea pigs cochlea

Objective To study the effects of ligustrazine(TMP) on gentamicin ototoxicity-induced expression of heat shock protein HSP70mRNA in stria vascularis of guinea pigs cochlea,and explore the protective role of ligustrazine on gentamicin ototoxicity.Methods Immunohistochemistry staining in situ hybridization and image quantitative analysis system,combined with auditory brainstem response(ABR) were applied in the study.Results The threshold of ABR of GM and TMP+GM were(36.55±6.13)dB and(21.09±4.50)dB,there was a signifficiant difference between them(P0.01).The average gray value of HSP70mRNA of GM and TMP+GM were(89.31±1.47)dB and(94.16±2.15)dB,there was a signifficiant difference between them(P0.01).ABR threshold shift was in high correlation with expression of HSP70mRNA in each group(r=-0.873 4 to-0.984 1;P0.001).Conclusions The transcription and translation of HSP70mRNA are different in the cells of stria vascularis.Ligustrazine can reduce expression of HSP70mRNA in stria vascularis of guinea pigs cochlea,alleviate GM ototoxicity,and ameliorate hearing function.