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2007•Chinese Heart JournalRequires access

Effect of Tetramethylpyrazine on vascular smooth muscle cell proliferation induced by thrombin

Qingzhi Chen

Open publisher page 1 citations

Abstract

AIM To investigate the effect of tetramethylpyrazine(TMP)on the proliferation of vascular smooth muscle cell(VSMC)induced by thrombin and the possible underlying mechanism.METHODS VSMC were isolated from rabbit aorta using enzyme digestion method.WST-8 colorimetry was carried out to evaluate the cell growing state.Cell cycle analysis was evaluated by flow cytometry and immunocytochemical staining was used to study the expression of c-fos.RESULTS TMP inhibited the proliferation of VSMC induced by thrombin in a dose-dependent manner.20 mg/L TMP could delay the transition from G_ 0/G_ 1 phase into S phase and decrease the expression of c-fos induced by thrombin.CONCLUSION TMP inhibit the proliferation of VSMC induced by thrombin in a dose-dependent manner,at least in part by inhibiting the expression of c-fos induced by thrombin and preventing the transition from G_ 0/G_ 1 into S phase of VSMC.

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AIM To investigate the effect of tetramethylpyrazine(TMP)on the proliferation of vascular smooth muscle cell(VSMC)induced by thrombin and the possible underlying mechanism.METHODS VSMC were isolated from rabbit aorta using enzyme digestion method.WST-8 colorimetry was carried out to evaluate the cell growing state.Cell cycle analysis was evaluated by flow cytometry and immunocytochemical staining was used to study the expression of c-fos.RESULTS TMP inhibited the proliferation of VSMC induced by thrombin in a dose-dependent manner.20 mg/L TMP could delay the transition from G_ 0/G_ 1 phase into S phase and decrease the expression of c-fos induced by thrombin.CONCLUSION TMP inhibit the proliferation of VSMC induced by thrombin in a dose-dependent manner,at least in part by inhibiting the expression of c-fos induced by thrombin and preventing the transition from G_ 0/G_ 1 into S phase of VSMC.

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Available abstract

AIM To investigate the effect of tetramethylpyrazine(TMP)on the proliferation of vascular smooth muscle cell(VSMC)induced by thrombin and the possible underlying mechanism.METHODS VSMC were isolated from rabbit aorta using enzyme digestion method.WST-8 colorimetry was carried out to evaluate the cell growing state.Cell cycle analysis was evaluated by flow cytometry and immunocytochemical staining was used to study the expression of c-fos.RESULTS TMP inhibited the proliferation of VSMC induced by thrombin in a dose-dependent manner.20 mg/L TMP could delay the transition from G_ 0/G_ 1 phase into S phase and decrease the expression of c-fos induced by thrombin.CONCLUSION TMP inhibit the proliferation of VSMC induced by thrombin in a dose-dependent manner,at least in part by inhibiting the expression of c-fos induced by thrombin and preventing the transition from G_ 0/G_ 1 into S phase of VSMC.

Key concepts: Tetramethylpyrazine, Thrombin, Vascular smooth muscle, Flow cytometry, Cell growth, Chemistry, Cell, Cell cycle

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