Effect of Malachite Green on Mitosis of Vicia faba Root Tip Cell

[Objective] The research aimed to study the effect of malachite green(MG) on mitosis of Vicia faba root tip cell.[Method] Different concentrations of malachite green were used to treat Vicia faba root tip cell for 12,24,36,48 and 72 h.The mitosis of Vicia faba root tip cell was observed by conventional chromosome compression technology,and the mitotic index(MI) of each treatment group was counted.[Result] As the treated time prolonged,MI presented the M-shaped variation trend,which illustrated that Vicia faba root tip had a adaptive process to MG.As the concentration of MG increased,MI gradually rose.When the concentration of MG was during 0.50-1.00 mg/L,MI was the maximum.When the concentration of MG further increased,MI decreased.It showed that MG was favorable for the plant growth in the certain concentration range.[Conclusion] The research could provide the basis for the reasonable utilization of MG and the reference for evaluating the environmental harm of MG.