Establish Fingerprinting for the Main Inbred Lines of Shanxi Province by SSR Markers
Xue Jiquan
Abstract
Xue Jiquan
Abstract
23 maize inbred lines in shananxi province were analyzed by SSR marker.Among 100 pairs of primers,30 pairs with steady polymorphism were selected as a set of core primers.3 alleles to 8 alleles were detected by each pair primers.There were 153 alleles detected in total.The average number of alleles was 5.01 per pair primer,the PIC ranged from 0.524 to 0.884.We also established finger printing for the 23 maize inbred lines.The experiment showed that SSR technique was practical and effective in maize inbred lines and variety protection,authenticity and purity identification.
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23 maize inbred lines in shananxi province were analyzed by SSR marker.Among 100 pairs of primers,30 pairs with steady polymorphism were selected as a set of core primers.3 alleles to 8 alleles were detected by each pair primers.There were 153 alleles detected in total.The average number of alleles was 5.01 per pair primer,the PIC ranged from 0.524 to 0.884.We also established finger printing for the 23 maize inbred lines.The experiment showed that SSR technique was practical and effective in maize inbred lines and variety protection,authenticity and purity identification.
Key concepts: Inbred strain, Allele, Biology, Primer (cosmetics), Genetics, Polymorphism (computer science), Gene, Physics