The SSR Markers Analysis of 60 Maize Inbred Lines
Ma Yuguang
Abstract
Ma Yuguang
Abstract
Simple sequence repeats(SSRs)markers were used to detect genetic variation of 60 elite maize inbred lines.Twenty-three SSR primers giving stable amplified detected 96 alleles among the inbred lines tested.The average number of allele per SSR locus was 3.96 with an range from 2 to 7.The value of polymorphism information content(PIC)for each SSR locus varied from 0.15 to 0.80 with an average of 0.600.The UPGMA analysis classified 60 inbred lines into 6 clusters,which corresponed to the heterotic groups determined by their pedigree information and breeder′s experiences(i.e.Tangsipingtou,Luda Red Cob,PN,BSSS,Lancaster,Suwan)
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Simple sequence repeats(SSRs)markers were used to detect genetic variation of 60 elite maize inbred lines.Twenty-three SSR primers giving stable amplified detected 96 alleles among the inbred lines tested.The average number of allele per SSR locus was 3.96 with an range from 2 to 7.The value of polymorphism information content(PIC)for each SSR locus varied from 0.15 to 0.80 with an average of 0.600.The UPGMA analysis classified 60 inbred lines into 6 clusters,which corresponed to the heterotic groups determined by their pedigree information and breeder′s experiences(i.e.Tangsipingtou,Luda Red Cob,PN,BSSS,Lancaster,Suwan)
Key concepts: UPGMA, Inbred strain, Locus (genetics), Microsatellite, Genetics, Biology, Allele, Genetic variation