2011•Xiandai shengwu yixue jinzhanRequires access
Expression of VEGF-C and VEGFR-3 in gastric carcinoma and its significance
Jianhua Wei
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Abstract
Objective:To investigate the expression of vascular endothelial growth factor C(VEGF-C) and Vascular endothelial growth factor receptor-3(VEGFR-3) in gastric cancer,so that to determine the molecular markers in prognosis of gastric cancer.Methods:Collecting clinical data methods,Real-time PCR and ELISA were used to detect the expression of VEGF-C and VEGFR-3 in 43 cases of gastric cancer.Results:43 cases of gastric cancer have different levels of VEGF-C and VEGFR-3.Real-time PCR results showed the VEGF-C and VEGFR-3 expression were 0.07±0.01 and 0.12±0.01,0.03±0.01 and 0.06±0.02 respectively in lymph node metastasis group and non-metastasis group in gastric cancer.,the difference was statistically significant when compared with the control group(p 0.05).ELISA examination demonstrated that expressions of VEGF-C and VEGFR-3 protein in the gastric cancer group without lymph node metastasis and the gastric cancer group complicated by lymph node metastasis were obviously increased when compared with that in normal gastric tissue.Conclusions:VEGF-C and VEGFR-3 expression were closely related with lymph node metastasis,indicating that VEGF-C and VEGFR-3 detection can be used as molecular markers in prognosis of gastric cancer.