2004•Traditional Chinese Drug Research and Clinical PharmacologyRequires access

Determination of Cinnamic Acid in Cortex Cinnamomi by RP-HPLC

XU Hong-hua

Abstract

Objective To establish the RP-HPLC method for the determination of cinnamic acid in different parts of Cortex Cinnamomi which comes from Wulong and Xinyi in Guangdong province. Methods The samples were assayed on a DiamonsilTM C18 column(250 mm×4.6 mm,5 μm)with temperature at 40℃. The mobile phase was aqueous acetonitrile-0.01 %phosphoric acid (gradient elution); flow rate was 1 mL/min and the detection wavelength was at 278 nm. Results The contents of cinnamic acid in one-year branches were the highest in the samples from the two differents habitats.Conclusion The method is simple,reliable,repeatable,and is suitable for the determination of cinnamic acid in Cortex Cinnamomi.

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Key concepts: Cinnamic acid, Phosphoric acid, Chromatography, High-performance liquid chromatography, Chemistry, Gradient elution, Acetonitrile, Cortex (anatomy)