Immunomdulating effects of bamboo shoots polysaccharides on immunosuppressed Eriocheir sinensis

To observe the effects of bamboo shoots polysaccharides(BSP)on the immune functions of immunosuppressed Eriocheir sinensis,the crabs were assigned to three groups,the control group and the immunosuppressed model group fed with basic feed,and the polysaccharide regulation group fed with the feed of BSP(2 g/kg basic feed).The crabs in the model group and the polysaccharide group were injected with 10 mg/kg body weight of cyclophosphamide very 5days for three times,while the crabs in the control group were injected with sterile saline with the same method.The total haemocyte counts(THC),respiratory burst(RB)activity in haemocytes,phenoloxidase(PO),lysozyme(LSZ)and nitrie oxide synthase(NOS)activity in serum were examined at 0,3,6 and 12 d after the crabs in the polysaccharide group fed with the polysaccharide.The results showed that the THC,PO,LSZ and NOS activity in serum of the polysaccharide group were significant higher than those of the model group(P 0.05).However,no significant differences were observed in the RB activity of haemocyte between the model group and the polysaccharide group(P 0.05).It was concluded that the BSP could effectively regulate the immune function of immunosuppressed E.sinensis induced by cyclophosphamide.