Immunoregulation of Fuling Polysaccharide on Non-specific Immune Function in Soft-shelled Turtles(Trionyx sinensis) by Oral Administration

The effect of the dietary fuling polysaccharide on non-specific immune function in soft-shelled turtle(Trionyx sinensis)was studied.The turtles were divided into 3 groups : fuling polysaccharide group(fed with diet supplemented with 20.0 mg/kg fuling polysaccharide), yeast cell wall group(fed with diet supplemented with 500.0 mg/kg yeast cell wall), and control group( fed with basic diet).28 days later,the turtles were blooded,and the phagocytic activity of the leucocytes,serum lysozyme activity and complement activity were determined.The results showed that the phagocytic activity and lysozyme activity in fuling polysaccharide group and yeast cell wall group were significantly higher than the control group (t-test,P0.05).However,there were no significant differences between fuling polysaccharide group and yeast cell wall group (t-test,P0.05).The highest complement activity were observed in fuling polysaccharide group and yeast cell wall group,which were significantly higher than the control group(t-test,P0.05).As the complement activity was concerned,there were no significant differences between fuling polysaccharide group and yeast cell wall group(t-test,P0.05).The results demonstrated that the best supplementation ratio of the fuling polysaccharide in the soft-shelled turtle was about 2.0 mg/kg.