Changes of NGAL and KIM-1 in the early period of acute kidney injury by lipopolysaccharide-induced in rats

Objective To explore early changes of neutrophil gelatinase associated lipocalin(NGAL) and kideny injury molecule-1(KIM-1) in lipopolysaccharide(LPS)-induced acute kidney injury(AKI) in rats.Methods Thirty-two male Wistar rats were randomized to receive caudal injection of 1 ml normal saline(control group),or 5mg/kg LPS(LPS2,4,6 hour group).After the prescribed time,the blood and urine levels of NGAL and the urine level of KIM-1 were determined with enzyme-linked immunosorbent assay(ELISA),serum creatinine(SCr) with Jaffe's reaction and the morphology of kidneys observed under the optical microscope.Results Compared with normal control group,the level of Scr was elevated slightly in the LPS2h group(P 0.05),significantly in the LPS4h,6h group(P 0.01);the levels of the blood and urine of NGAL and urine of KIM-1 were significantly elevated in the LPS2,4,6h group compared with normal control group(P 0.01);Compared with LPS2h group,the levels of the blood and urine of NGAL were slightly increased in LPS4h group(P 0.05),but the KIM-1 were significantly elevated(P 0.01).HE staining showed the nephridial tissue was normal,whereas the nephridial tissue changed gradually in the LPS 2,4,6h group.Conclusion NGAK and KIM-1 were increased in the early period of AKI and may be used as a marker for early diagnosis;the KIM-1 had time dependence and may be used as a monitor marker of AKI.