Establishment of homozygote transgenic mice expressing SV40T in parietal cells

Aim:To establish homozygote mouse strains which express SV40T in parietal cells.Methods:The original template capacity between the known heterozygote mice and the unknown mice were compared by using real-time PCR to ensure their genotype, and then the results were validated by using cross test.Results: A total of 15 homozygous mice were selected by real-time PCR. The offspring of the homozygote mice and wild mice were all positive, the results of cross test were consistent with the real-time PCR.A total of 6 homozygote mouse strains had been established by mating among the homozygous compatriots.Conclusion: The real-time PCR is a speedy,convenient method with high-flux,hence could be used to estimate the genotype of transgenic mice.