Identification of transgenic mice with Spyda gene over expression

Objective:To establish transgenic mice with Spyda gene over-expression to study the function of Spyda gene.Methods:The mice with Spyda gene over-expression were obtained by microinjecting the Spyda expression vector which was established by Gateway technology.After three generations,the levels of original template detected by real-time fluorescent quantitative PCR were compared between the heterozygous and homozygous mice and the results were validated by using cross test.Results:Seven Spyda gene over-expression mice were obtained,and four homozygous mice were screened by real-time fluorescent quantitative PCR.The results of cross test indicated that the offspring of the homozygous mice mated with normal mice were all positive.These were consistent with the results of real-time fluorescent quantitative PCR.Two independent homozygous transgenic mouse strains had been established.Conclusions:Spyda gene over-expression transgenic mouse strains had been established.The homozygous transgenic mouse is a tool to study the function of Spyda gene.