Cloning and Tissue Expression of Mx1 Gene in ICR Mouse

This study was carried out to obtain and analyze sequence of ICR mouse Mx1 gene,and to investigate the gene expression patterns of multiple tissues.Full-length sequence of ICR mouse Mx1 gene was amplified by RT-PCR from the total RNA extracted from mouse peritoneal macrophages induced by poly(I) /(C) and its expression in different tissues was studied by semi-quantity RT-PCR.A 1 611 bp cDNA of Mx1 gene encoding 530 amino acids was first cloned from fetal ICR mouse peritoneal macrophages.Analysis of homology showed that the mouse Mx1 gene and the deduced amino acids shared 37.36 % to 69.98% and 38.8% to 76.6% homologies with those from other animals.The semi-quantity RT-PCR revealed ICR mouse Mx1 gene nucleotide sequence displayed high expression in spleen and lung,middle expression in liver,low expression in heart and kidney,no expression in muscle.Mx protein coded by the amplified mouse Mx1 gene had the common characteristics of vertebrates' Mx proteins.The expression level of Mx1 gene is particular to tissues.