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2002•Journal of Radiation Research and Radiation ProcessingRequires access

EFFECTS OF ~(60) Co γ RAYS ON THE CELL CYCLE PROGRESS OF MCF-7 CELLS

Yang Yepeng

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Abstract

To investigate the effects of ionizing radiation on cell cycle progress of tumor cell lines, the human breast cancer MCF-7 cell line cultured in vitro was exposed to 60 Co γ rays and the alterations in cell cycle progress after irradiation were measured by flow cytometry. The results indicated that the MCF-7 cells showed a transient S arrest continuing for about 6h and an obvious G 2 arrest continuing for about 63h after irradiation with 5.0Gy γ rays. S and G 2 arrest culminated at 9h and 18h respectively after irradiation and the peak values of S and G 2 arrest reached respectively 1.6 times and 6.2 times as many as normal value. The dose-effect curve examined 9h after irradiation was quite different from that examined 18h after irradiation. Both of the S arrest at 9h after irradiation and the G 2 arrest at 18h after irradiation presented significant relationship with irradiation dose.

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What this paper is about

To investigate the effects of ionizing radiation on cell cycle progress of tumor cell lines, the human breast cancer MCF-7 cell line cultured in vitro was exposed to 60 Co γ rays and the alterations in cell cycle progress after irradiation were measured by flow cytometry. The results indicated that the MCF-7 cells showed a transient S arrest continuing for about 6h and an obvious G 2 arrest continuing for about 63h after irradiation with 5.0Gy γ rays. S and G 2 arrest culminated at 9h and 18h respectively after irradiation and the peak values of S and G 2 arrest reached respectively 1.6 times and 6.2 times as many as normal value. The dose-effect curve examined 9h after irradiation was quite different from that examined 18h after irradiation. Both of the S arrest at 9h after irradiation and the G 2 arrest at 18h after irradiation presented significant relationship with irradiation dose.

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Available abstract

To investigate the effects of ionizing radiation on cell cycle progress of tumor cell lines, the human breast cancer MCF-7 cell line cultured in vitro was exposed to 60 Co γ rays and the alterations in cell cycle progress after irradiation were measured by flow cytometry. The results indicated that the MCF-7 cells showed a transient S arrest continuing for about 6h and an obvious G 2 arrest continuing for about 63h after irradiation with 5.0Gy γ rays. S and G 2 arrest culminated at 9h and 18h respectively after irradiation and the peak values of S and G 2 arrest reached respectively 1.6 times and 6.2 times as many as normal value. The dose-effect curve examined 9h after irradiation was quite different from that examined 18h after irradiation. Both of the S arrest at 9h after irradiation and the G 2 arrest at 18h after irradiation presented significant relationship with irradiation dose.

Key concepts: Irradiation, MCF-7, Ionizing radiation, Cell cycle checkpoint, Cell cycle, Flow cytometry, Cell culture, Radiochemistry

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EFFECTS OF ~(60) Co γ RAYS ON THE CELL CYCLE PROGRESS OF MCF-7 CELLS — Research Paper | ScholarLens