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2013•Zhongguo shiyan fangjixue zazhiRequires access

HPLC Determination of Quercetin and Kaempferol in Ailanthi Fructus

Meng Xian-bo

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Abstract

Objective:To establish an HPLC method for the simultaneous determination of quercetin and kaempferol in Ailanthi Fructus.Method:Quercetin and kaempferol were separated on Inertsil ODS-3(4.6 mm×250 mm,5 μm) column and detected at 360 nm.The mobile phase was methanol-0.4% phosphate(50∶50).The flow rate was 1.0 mL·min-1.Result:Quercetin and kaempferol were linear within the range of 11.1-333 μg(r=1),2.3-69 μg(r=1) respectively.The average recovery was 97.82% with RSD of 1.1%,96.81% with RSD of 1.7% respectively.Conclusion:The method is simple,reproducible,and it can be used for the quality control of Ailanthi Fructus.

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Objective:To establish an HPLC method for the simultaneous determination of quercetin and kaempferol in Ailanthi Fructus.Method:Quercetin and kaempferol were separated on Inertsil ODS-3(4.6 mm×250 mm,5 μm) column and detected at 360 nm.The mobile phase was methanol-0.4% phosphate(50∶50).The flow rate was 1.0 mL·min-1.Result:Quercetin and kaempferol were linear within the range of 11.1-333 μg(r=1),2.3-69 μg(r=1) respectively.The average recovery was 97.82% with RSD of 1.1%,96.81% with RSD of 1.7% respectively.Conclusion:The method is simple,reproducible,and it can be used for the quality control of Ailanthi Fructus.

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Available abstract

Objective:To establish an HPLC method for the simultaneous determination of quercetin and kaempferol in Ailanthi Fructus.Method:Quercetin and kaempferol were separated on Inertsil ODS-3(4.6 mm×250 mm,5 μm) column and detected at 360 nm.The mobile phase was methanol-0.4% phosphate(50∶50).The flow rate was 1.0 mL·min-1.Result:Quercetin and kaempferol were linear within the range of 11.1-333 μg(r=1),2.3-69 μg(r=1) respectively.The average recovery was 97.82% with RSD of 1.1%,96.81% with RSD of 1.7% respectively.Conclusion:The method is simple,reproducible,and it can be used for the quality control of Ailanthi Fructus.

Key concepts: Kaempferol, Quercetin, Chromatography, Chemistry, High-performance liquid chromatography, Biochemistry, Antioxidant

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